TLC Silica gel 60 RP-2 F254 (silanized) 25 Glass plates 20 x 20 cm

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/gPore volume (N₂-isotherm): 0.74 - 0.84 ml/gd 50 (laser diffraction, size distribution): 13 - 14 µmLayer thickness: 190 - 250 µmDeviation of layer thickness per plate: ≤ 40 µmChromatographic testings: A) Cholesterin testhRf-values - cholesterin, cholesterin test: 80 - 90B) Steroid test - methyltestosterone, steroid test: 35 - 44 - Reichstein S, steroid test: 46 - 54 - hydrocortisone, steroid test: 53 - 61Typical value determined on an activated plate (120°C, 15 min) Eluent a) aceton/ water (95/5 v/v) Eluent b) aceton/ water (60/40 v/v)

General description

Glass TLC plates, RP-2 modified silica gel coated with flourescent indicator F254. The RP-2 modified silica gel TLC plates come in a quantity of 25 size 20x20 cm for thin layer chromatography applications.

RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What's more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Linkage

Replaces: 5747-7; 5747